Title : Molecular cloning of a glycosylphosphatidylinositol-anchored molecule
CDw108
Abstract :
- CDw108 , also known as the John-Milton-Hagen human blood group Ag, is an 80-kDa glycosylphosphatidylinositol ( GPI )-anchored membrane glycoprotein that is preferentially expressed on activated lymphocytes and E
- The molecular characteristics and biological function of the CDw108 were not clarified previously
- In this manuscript, we identify the cDNA clone containing the entire coding sequence of the CDw108 gene and report its molecular characteristics
- The 1998-base pairs of the open reading frame of the cloned cDNA encoded a protein of 666 amino acids (aa), including the 46 aa of the signal peptide and the 19 aa of the GPI-anchor motif
- Thus, the membrane-anchoring form of CDw108 was the 602 aa, and the estimated molecular mass of the unglycosylated form was 68 kDa
- The RGD (Arg-Gly-Asp) cell attachment sequence and the five potential N-linked glycosylation sites were located on the membrane-anchoring form
- Flow cytometric and immunoprecipitation analyses of the CDw108 cDNA transfectants confirmed that the cloned cDNA encoded the native form of CDw108
- The CDw108 mRNA was expressed in activated PBMCs as well as in the spleen, thymus, testis, placenta, and brain, but was not expressed in any other tissues tested
- Radiation hybrid mapping indicated that the CDw108 gene was located in the middle of the long arm of chromosome 15 (15q23-24)
- This molecular information will be critical for understanding the biological function of the CDw108 Ag