Title : Structure of the oligosaccharide of human J chain
Abstract :
The complete structure of the oligosaccharide moiety of J chain isolated from a Waldenströms macroglobulin Wa has been established
The oligosaccharide is present in three forms differing in the amount of sialic acid and designated J-A, J-B, and J-C
The structure and proportion of each of these is: formula : (see text) : Removal of the oligosaccharide moiety with glycosidases results in an increased mobility of J chain in sodium dodecyl sulfate polyacrylamide gel electrophoresis corresponding to a shift in apparent molecular weight from 23,500 to 19,500
The preparation and utilization of iodinated glycopeptides for sequence analysis is presented