Title : A novel
Ser O-glucuronidation in acidic
proline-rich
proteins identified by tandem mass spectrometry
Abstract :
- Human acidic proline-rich salivary protein PRP-1 and its C-terminally truncated form PRP-3 were analyzed by electrospray tandem mass spectrometry
- Post-translational modifications were detected and characterized
- A pyroglutamic acid residue was demonstrated at the N-terminus, Ser-8 and Ser-22 were shown to be phosphorylated and an O-linked glucuronic acid conjugation was identified
- The latter modification was located to Ser-17 and found to be present in approximately 40% of the polypeptides