PMID: 10858503

 

    Legend: Sugar

Title : A novel Ser O-glucuronidation in acidic proline-rich proteins identified by tandem mass spectrometry

Abstract :
  1. Human acidic proline-rich salivary protein PRP-1 and its C-terminally truncated form PRP-3 were analyzed by electrospray tandem mass spectrometry
  2. Post-translational modifications were detected and characterized
  3. A pyroglutamic acid residue was demonstrated at the N-terminus, Ser-8 and Ser-22 were shown to be phosphorylated and an O-linked glucuronic acid conjugation was identified
  4. The latter modification was located to Ser-17 and found to be present in approximately 40% of the polypeptides