Title :
Reprimo , a new candidate mediator of the
p53-mediated cell cycle arrest at the G2 phase
Abstract :
- A novel gene, Reprimo , in which induction in cells exposed to X-irradiation is dependent on p53 expression, has been isolated
- Ectopic p53 expression results in the induction of its mRNA
- Reprimo is a highly glycosylated protein and, when ectopically expressed, it is localized in the cytoplasm and induces G(2) arrest of the cell cycle
- In the arrested cells, both Cdc2 activity and nuclear translocation of cyclin B1 are inhibited, suggesting the involvement of Reprimo in the Cdc2 . cyclin B1 regulation pathway
- Thus, Reprimo may be a new member involved in the regulation of p53-dependent G(2) arrest of the cell cycle