Title : Structural analysis of sulfated N-linked oligosaccharides in erythropoietin
Abstract :
We previously demonstrated that high-performance liquid chromatography with electrospray ionization mass spectrometry (LC/MS) equipped with a graphitized carbon column ( GCC ) is useful for the structural analysis of carbohydrates in glycoproteins
Using LC/MS with GCC , sulfated N-linked oligosaccharides were found in erythropoietin ( EPO ) expressed in baby hamster kidney cells
Sulfation occurs in a part of the N-linked oligosaccharides in the EPO
Sulfated monosaccharide residue in the sulfated N-linked oligosaccharide residue in the sulfated N-linked oligosaccharide was determined by exoglycosidase digestion followed by sugar mapping by LC/MS
The linkage position and branch-location of the sulfate group in the tetraantennary oligosaccharide were analyzed by (1)H-nuclear magnetic resonance
It was suggested that sulfation occurs on the C-6 position of GlcNAc located in the GlcNAcbeta1-4Manalpha1-3 branch