Title : N-glycosylation potential of maize: the human
lactoferrin used as a model
Abstract :
- In order to determine the N-glycosylation potential of maize, a monocotyledon expression system for the production of recombinant glycoproteins , human lactoferrin was used as a model
- The human lactoferrin coding sequence was inserted into the pUC18 plasmid under control of the wheat glutenin promoter
- Maize was stably transformed and recombinant lactoferrin was purified from the fourth generation seeds
- Glycosylation was analysed by gas chromatography, lectin detection, glycosidase digestions and mass spectrometry
- The results indicated that both N-glycosylation sites of recombinant lactoferrin are mainly substituted by typical plant paucimannose-type glycans, with beta1,2-xylose and alpha1,3-linked fucose at the proximal N-acetylglucosamine, and that complex-type glycans with Lewis(a) determinants are not present in maize recombinant lactoferrin