Title : A proteomic analysis of human bile
Abstract :
- We have carried out a comprehensive characterization of human bile to define the bile proteome
- Our approach involved fractionation of bile by one-dimensional gel electrophoresis and lectin affinity chromatography followed by liquid chromatography tandem mass spectrometry
- Overall, we identified 87 unique proteins , including several novel proteins as well as known proteins whose functions are unknown
- A large majority of the identified proteins have not been previously described in bile
- Using lectin affinity chromatography and enzymatically labeling of asparagine residues carrying glycan moieties by (18)O , we have identified a total of 33 glycosylation sites
- The strategy described in this study should be generally applicable for a detailed proteomic analysis of most body fluids
- In combination with "tagging" approaches for differential proteomics, our method could be used for identification of cancer biomarkers from any body fluid