Title : Beta-amyloid
protein converting
enzyme 1 1 and brain-specific type II membrane
protein BRI3 : binding partners processed by
furin
Abstract :
- Using a yeast two-hybrid system, we screened a human brain cDNA library for possible interacting proteins with the C-terminal cytosolic tail of the beta-secretase beta-amyloid protein converting enzyme ( BACE)1
- This identified seven potential candidates, including the brain-specific type II membrane protein BRI3
- Co-localization and co-immunoprecipitation experiments confirmed that BACE1 and BRI3 co-localize and interact with each other via the cytosolic tail of BACE1
- Furthermore, pulse and pulse-chase analyses revealed that the pro- protein convertases furin , and to a lesser extent PC7 and PC5A, process BRI3 into a C-terminal secreted approximately 4-kDa product
- Thus, furin efficiently processes both pro- BACE1 and its novel interacting protein pro- BRI3