Title : Roles for N-glycosylation in the dynamics of
Edg-1 /
S1P1 in sphingosine 1-phosphate-stimulated cells
Abstract :
- Sphingosine 1-phosphate ( Sph-1-P) is a bioactive lipid mediator released from activated platelets
- To date, 5 seven-transmembrane-spanning receptors , Edg-1 / S1P1 , Edg-3 / S1P3 , Edg-5 / S1P2 , Edg-6 / S1P4 and Edg-8 / S1P5 , have been identified as specific Sph-1-P receptors
- Our recent novel studies established that Edg-1 / S1P1 is glycosylated in its N-terminal extracellular portion and further identified the specific glycosylation site as asparagine 30
- We also demonstrated that the structure of the N-terminal ectodomain of Edg-1 / S1P1 affects both its transport to the cell surface and the N-glycosylation process
- These studies revealed a possible regulatory role for the N-glycan on Edg-1 / S1P1 in the dynamics of the receptor, such as its lateral and internal movements within the membrane, in ligand-stimulated mammalian cells