PMID: 15750791

 

    Legend: Sugar

Title : Roles for N-glycosylation in the dynamics of Edg-1 / S1P1 in sphingosine 1-phosphate-stimulated cells

Abstract :
  1. Sphingosine 1-phosphate ( Sph-1-P) is a bioactive lipid mediator released from activated platelets
  2. To date, 5 seven-transmembrane-spanning receptors , Edg-1 / S1P1 , Edg-3 / S1P3 , Edg-5 / S1P2 , Edg-6 / S1P4 and Edg-8 / S1P5 , have been identified as specific Sph-1-P receptors
  3. Our recent novel studies established that Edg-1 / S1P1 is glycosylated in its N-terminal extracellular portion and further identified the specific glycosylation site as asparagine 30
  4. We also demonstrated that the structure of the N-terminal ectodomain of Edg-1 / S1P1 affects both its transport to the cell surface and the N-glycosylation process
  5. These studies revealed a possible regulatory role for the N-glycan on Edg-1 / S1P1 in the dynamics of the receptor, such as its lateral and internal movements within the membrane, in ligand-stimulated mammalian cells