PMID: 15983417

 

    Legend: Sugar

Title : Structure of insect-cell-derived IL-22

Abstract :

1. The crystal structure of interleukin-22 expressed in Drosophila melanogaster S2 cells ( IL-22 ( Dm )) has been determined at 2.6 A resolution
2. IL-22 ( Dm ) crystals contain six molecules in the asymmetric unit
3. Comparison of IL-22 ( Dm ) and IL-22 ( Ec ) ( interleukin-22 produced in Escherichia coli) structures reveals that N-linked glycosylation causes only minor structural changes to the cytokine
4. However, 1-4 A main-chain differences are observed between the six IL-22 ( Dm ) monomers at regions corresponding to the IL-22R1 and IL-10R2 binding sites
5. The structure of the carbohydrate and the conformational variation of IL22 ( Dm ) provide new insights into IL-22 receptor recognition