PMID: 16107205

 

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Title : Physical and biochemical properties of mammalian DNase X proteins : non-AUG translation initiation of porcine and bovine mRNAs for DNase X . DNase X is the first human DNase protein identified as being homologous with DNase I

Abstract :
  1. In the present study we describe the isolation of several mammalian DNase X cDNAs and the molecular characterization of their coding proteins
  2. A sequence comparison reveals some conserved characteristics: all the mammalian DNase X proteins have an N-terminal signal peptide , a potential N-linked glycosylation site and a C-terminal hydrophobic domain
  3. Human DNase X , ectopically expressed in HeLa S3 cells, is located in the ER (endoplasmic reticulum) and is modified by an N-linked glycosylation at Asn-243
  4. Gene expression analyses show that the high expression level in muscular tissues, a known feature of human DNASE X , is also observed in mouse DNase X . Interestingly, the translation of porcine and bovine DNase X proteins occurs in the absence of an in-frame AUG initiation codon
  5. We show that their mRNAs utilize a conserved CUG triplet for translation initiation