Title : Identification of
N-linked glycoproteins in human saliva by
glycoprotein capture and mass spectrometry
Abstract :
- Glyco proteins make up a major and important part of the salivary proteome and play a vital role in maintaining the health of the oral cavity
- Because changes in the physiological state of a person are reflected as changes in the glycoproteome com position, mapping the salivary glycoproteome will provide insights into various processes in the body
- Salivary glycoproteins were identified by the hydrazide coupling and release method
- In this approach, glycoproteins were coupled onto a hydrazide resin, the proteins were then digested and formerly N-glycosylated peptides were selectively released with the enzyme PNGase F and analyzed by LC-MS/MS
- Employing this method, coupled with in-solution isoelectric focusing separation as an additional means for pre-fractionation, we identified 84 formerly N-glycosylated peptides from 45 unique N-glycoproteins
- Of these, 16 glycoproteins have not been reported previously in saliva
- In addition, we identified 44 new sites of N-linked glycosylation on the proteins