Title :
Nectin-like molecule 1 is a
glycoprotein with a single N-glycosylation
site at N290KS which influences its adhesion activity
Abstract :
- Nectin-like molecule 1 ( NECL1 )/ CADM3 / IGSF4B / TSLL1 / SynCAM3 , from now on referred to as NECL1 , is a neural tissue-specific immunoglobulin-like cell-cell adhesion molecule which has Ca(2 +)-independent homo- or heterophilic cell-cell adhesion activity and plays an important role in the formation of synapses, axon bundles and myelinated axons
- Here we first detected the expression of NECL1 in human fetal and adult brains, and mouse brains at different developmental stages
- The results indicated that two bands with molecular weights of about 62 kDa and 48 kDa were found in human fetal brain, while only one band with a molecular weight of about 48 kDa was found in human adult brain; two bands with molecular weights of about 62 kDa and 48 kDa whose expression level gradually increased were also found from mouse E16 to P14 , while only one band with a molecular weight of about 48 kDa was found from P14
- Bioinformatics analysis showed there were two putative N-glycosylation sites within human NECL1 at positions N25LS and N290KS and within mouse Necl1 at positions N23LS and N288KS, respectively
- There was no O-glycosylation site in either human NECL1 or mouse Necl1
- Based on the results of N-Glycosidase F treatment with human fetal brain tissue and lysates from transient transfection with human wild-type or glycosylation site mutant NECL1 in 293ET cells, we demonstrated that human NECL1 is an N-linked glycoprotein with a single glycosylation site at position N290KS
- Cell aggregation assay further showed there was an increased adhesion activity after the glycosylation site mutation of NECL1 molecule