Title : Simultaneous glycosylation analysis of human serum
glycoproteins by high-performance liquid chromatography/tandem mass spectrometry
Abstract :
- Changes in the glycosylation of some serum proteins are associated with certain diseases
- In this study, we performed simultaneous site-specific glycosylation analysis of abundant serum glycoproteins by LC/Qq-TOF MS of human serum tryptic digest, the albumin of which was depleted
- The glycopeptide peaks on the chromatogram were basically assigned by database searching with modified peak-list text files of MS/MS spectra and then based on mass differences of glycan units from characterized glycopeptides
- Glycopeptide of IgG , haptoglobin and ceruloplasmin were confirmed by means of a comparison of their retention times and m/z values with those obtained by LC/MS of commercially available glycoproteins
- Mass spectrometric carbohydrate heterogeneity in the assigned glycopeptides was analyzed by an additional LC/MS
- We successfully demonstrated site-specific glycosylation of 23 sites in abundant serum glycoproteins