Title : Carbohydrate analysis of human
von Willebrand factor with horseradish peroxidase-conjugated lectins
Abstract :
- Human von Willebrand factor ( vWF ) immobilized on a polyvinylidene difluoride membrane was subjected to binding assay with a series of horseradish peroxidase-conjugated lectins
- The protein was reactive with concanavalin A , Ricinus communis agglutinin 120 , wheat germ agglutinin and Ulex europaeus agglutinin I (UEA-I) but not with peanut agglutinin before sialidase treatment
- These reactivities were consistent with the major oligosaccharide structure reported except for UEA-I
- The reactivity with UEA-I was greatly decreased after digestion of the protein with either alpha-L-fucosidase or peptide-N-glycosidase F , but no significant decrease was observed after mild alkaline treatment or delipidation
- vWF and UEA-I have been independently used as a good marker for human endothelial cells
- Our results indicate that vWF itself contains UEA-I reactive sugar chains in its Asn-linked oligosaccharides. Asn-linked oligosaccharides