Title : Carbohydrate structure of human pancreatic elastase 1
Abstract :
Human pancreatic elastase 1 ( E1 ) is a glycoprotein containing two potential N-glycosylation sites , one of which carries a carbohydrate moiety [Wendorf , Geyer, Sziegoleit & Linder (1989) FEBS Lett
249, 275-278]
In order to study its glycosylation, glycoprotein isolated from post-mortem pancreas tissue of 75 donors was digested with trypsin
Oligosaccharides were liberated from resulting glycopeptides by treatment with peptide-N4-(N-acetyl-beta-glycosaminyl)- asparagine amidase F, radiolabelled by reduction with KB3H4 and separated by h.p.l.c. and gel filtration
Major oligosaccharide alditol fractions , representing 67.8 mol% of total glycans , were characterized by methylation analysis and sequential degradation with exoglycosidases
The results revealed that about two-fifths of the partially truncated, mainly biantennary, complex-type glycans found comprised blood group A, B, Lea (or X), difucosyl A or difucosyl B determinants , which could be assigned to lactosamine antennae linked to Man(alpha 1-3)- residues of the sugar chains