PMID: 2023947

 

    Legend: Sugar

Title : Characterization of a posttranslational fucosylation in the growth factor domain of urinary plasminogen activator

Abstract :
  1. A posttranslational modification site in natural and recombinant urinary-type plasminogen activators (urokinases; EC 3.4.21.31) has been localized to Thr-18 , in the growth factor domain of the molecule
  2. This is the region of urinary plasminogen activator responsible for its specific receptor binding
  3. An unusual carbohydrate-protein linkage, a single monosaccharide, fucose, covalently attached directly to threonine in the peptide , is described here
  4. The glycan moiety and the site of modification have been identified with mass spectrometry and confirmed by carbohydrate com position analysis, Edman degradation, and one- and two-dimensional NMR studies
  5. This type of modification is normally not detected without mass spectrometry because the fucose-threonine bond threonine bond is hydrolyzed under standard acidic conditions of the amino acid analysis and Edman sequencing
  6. This modification may be widely found in other proteins