PMID: 2129367

 

    Legend: Sugar

Title : A new trisaccharide sugar chain linked to a serine residue in the first EGF-like domain of clotting factors VII and IX and protein Z . Recently, we determined the complete amino acid sequence of bovine factor VII (Takeya, H. et al. (1988) J. Biol

Abstract :
  1. Chem
  2. 263, 14868-14877)
  3. In the course of the studies, we found an unknown serine derivative at position 52 in the first epidermal growth factor-like domain of factor VII
  4. A pentapeptide isolated from the S-aminoethylated factor VII contained Ser-52 , which could not be identified with a gas-phase sequencer
  5. The same results were also obtained for a pentapeptide containing Ser-53 of factor IX and protein Z . Component sugar analysis revealed that the peptide contained 1 mol of glucose and 2 mol of xylose.
  6. This sugar component was also confirmed by high-resolution fast atom bombardment mass spectrometric analysis of the pentapeptide
  7. The trisaccharide was released from the peptides by means of beta-elimination reaction and its reducing end was identified as pyridylamino-glucose.
  8. These results indicate the existence of a (Xyl2)Glc-Ser structure Ser structure in factors VII , IX and protein Z . Similar results were obtained for human factors VII , IX and protein Z
  9. This is the first report of a (Xyl2)-Glc-Ser structure Ser structure in glycoproteins to our knowledge
  10. The presence of the unique trisaccharide structure in factors VII , IX and protein Z leads us to anticipate its biological role in the tissue factor pathway