PMID: 22171320

 

    Legend: Sugar

Title : Human urinary glycoproteomics; attachment site specific analysis of N- and O-linked glycosylations by CID and ECD

Abstract :
  1. Urine is a complex mixture of proteins and waste products and a challenging biological fluid for biomarker discovery
  2. Previous proteomic studies have identified more than 2800 urinary proteins but analyses aimed at unraveling glycan structures and glycosylation sites of urinary glycoproteins are lacking
  3. Glycoproteomic characterization remains difficult because of the complexity of glycan structures found mainly on asparagine asparagine (N-linked) or serine/threonine (O-linked) residues
  4. We have developed a glycoproteomic approach that combines efficient purification of urinary glycoproteins/glycopeptides with complementary MS-fragmentation techniques for glycopeptide analysis
  5. Starting from clinical sample size, we eliminated interfering urinary compounds by dialysis and concentrated the purified urinary proteins by lyophilization
  6. Sialylated urinary glycoproteins were conjugated to a solid support by hydrazide chemistry and trypsin digested
  7. Desialylated glycopeptides , released through mild acid hydrolysis, were characterized by tandem MS experiments utilizing collision induced dissociation (CID) and electron capture dissociation fragmentation techniques
  8. In CID- MS(2 ), Hex ( 5)HexNAc (4)-N- Asn and HexHexNAc-O-Ser/Thr were typically observed, in agreement with known N-linked biantennary complex-type and O-linked core 1-like structures , respectively
  9. Additional glycoforms for specific N- and O-linked glycopeptides were also identified, e.g. tetra-antennary N-glycans and fucosylated core 2-like O-glycans.
  10. Subsequent CID-MS(3), of selected fragment-ions from the CID-MS(2 ) analysis, generated peptide specific b- and y-ions that were used for peptide identification
  11. In total, 58 N- and 63 O-linked glycopeptides from 53 glycoproteins were characterized with respect to glycan- and peptide sequences
  12. The combination of CID and electron capture dissociation techniques allowed for the exact identification of Ser/Thr attachment site(s) for 40 of 57 putative O-glycosylation sites
  13. We defined 29 O-glycosylation sites which have, to our knowledge, not been previously reported
  14. This is the first study of human urinary glycoproteins where "intact" glycopeptides were studied, i.e. the presence of glycans and their attachment sites were proven without doubt