PMID: 2226797

 

    Legend: Sugar

Title : Carbohydrate structures of a human tissue plasminogen activator variant expressed in recombinant Chinese hamster ovary cells

Abstract :

1. The carbohydrate structures of a genetically engineered human tissue plasminogen activator variant bearing a single N-glycosylation site at Asn 448 are reported
2. After isolation of the tryptic glycopeptide and liberation of the N-linked carbohydrates by polypeptide:N-glycosidase F , 6 major oligosaccharide fractions were separated by HPLC on NH2-bonded phase
3. Their structures were determined by com position al and methylation analyses combined with fast atom bombardment mass spectrometry
4. Seventy percent of the carbohydrates were of the biantennary complex type with fucose at the proximal GlcNAc and zero, one or two alpha 2-3 linked NeuAc.
5. The remainder were triantennary structures with one, two or three NeuAc.