Title : Determination of
cathepsin V activity and intracellular trafficking by N-glycosylation
Abstract :
- Cathepsin V ( L2 ), a lysosomal cysteine protease , is a member of cathepsin family, relating to cancer invasion and metastasis
- Cathepsin V contains two predicted N-glycosylation sites , but it has not been reported whether cathepsin V is glycosylated or not
- In this study, we clarified the role of N-glycosylation of cathepsin V for its functions
- We demonstrated that cathepsin V is N-glycosylated at both Asn(221) and Asn(292) using mass spectrometry and site-directed mutagenesis
- N-glycosylation of cathepsin V was important for transportation to lysosome, secretion, and activity in HT1080 cells
- These data demonstrated that functions of cathepsin V are controlled by N-glycosylation