PMID: 22967898

 

    Legend: Sugar

Title : Determination of cathepsin V activity and intracellular trafficking by N-glycosylation

Abstract :
  1. Cathepsin V ( L2 ), a lysosomal cysteine protease , is a member of cathepsin family, relating to cancer invasion and metastasis
  2. Cathepsin V contains two predicted N-glycosylation sites , but it has not been reported whether cathepsin V is glycosylated or not
  3. In this study, we clarified the role of N-glycosylation of cathepsin V for its functions
  4. We demonstrated that cathepsin V is N-glycosylated at both Asn(221) and Asn(292) using mass spectrometry and site-directed mutagenesis
  5. N-glycosylation of cathepsin V was important for transportation to lysosome, secretion, and activity in HT1080 cells
  6. These data demonstrated that functions of cathepsin V are controlled by N-glycosylation