PMID: 2550193

 

    Legend: Sugar

Title : Recombinant human erythropoietin produced by Namalwa cells

Abstract :
  1. To establish a practical exogenous gene expression system in human cells, a cDNA coding for human erythropoietin ( EPO ) was expressed in human B-lymphoblastoid Namalwa cells
  2. The Namalwa-derived recombinant EPO was purified from the culture fluid by a simple three-step procedure
  3. The Namalwa EPO showed an equivalent activity in vivo to that of human urinary EPO
  4. Oligosaccharide structure analyses suggested that almost all N-linked oligosaccharide chains of Namalwa EPO are shared by urinary EPO
  5. The two major N-linked oligosaccharides of Namalwa EPO were fucose-containing tetraantennary and fucose-containing triantennary structures