Title : Recombinant human
erythropoietin produced by Namalwa cells
Abstract :
- To establish a practical exogenous gene expression system in human cells, a cDNA coding for human erythropoietin ( EPO ) was expressed in human B-lymphoblastoid Namalwa cells
- The Namalwa-derived recombinant EPO was purified from the culture fluid by a simple three-step procedure
- The Namalwa EPO showed an equivalent activity in vivo to that of human urinary EPO
- Oligosaccharide structure analyses suggested that almost all N-linked oligosaccharide chains of Namalwa EPO are shared by urinary EPO
- The two major N-linked oligosaccharides of Namalwa EPO were fucose-containing tetraantennary and fucose-containing triantennary structures