Title :
GP73 N-glycosylation at
Asn144 reduces hepatocellular carcinoma cell motility and invasiveness
Abstract :
- Golgi Protein 73 ( GP73 ) is a potential liver disease glycobiomarker warranting comprehensive analyses of its glycan structure and glycosylation function
- In this study, we used mass spectrometry to identify glycosylation sites and the glycan structure , high-throughput lectin microarray to provide rapid and sensitive profiling of glycoconjugates, and site-directed mutagenesis to clarify the impact of glycans on target glycoproteins in vivo
- We identified three GP73 N-glycosylation sites : Asn109, Asn144 and Asn398
- We found five glycoforms on Asn144 , including biantennary, triantennary and fucosylated glycans
- Removal of N-glycans at Asn144 enhanced the motility and invasiveness of hepatocellular carcinoma cells, possibly due to inhibition of cell adhesion related to the changes of cell membrane glycosylation
- This study increases our understanding of the functional relevance of GP73 glycosylation and suggests that Asn144-deleted GP73 can influence the progression and metastasis of hepatocellular carcinoma