Title :
Nogo Receptor crystal structures with a native disulfide pattern suggest a novel mode of self-interaction
Abstract :
- The Nogo Receptor ( NgR ) is a glycophosphatidylinositol-anchored cell-surface protein and is a receptor for three myelin-associated inhibitors of regeneration: myelin-associated glycoprotein , Nogo66 and oligodendrocyte myelin glycoprotein
- In combination with different co-receptors , NgR mediates signalling that reduces neuronal plasticity
- The available structures of the NgR ligand-binding leucine-rich repeat (LRR) domain leucine-rich repeat (LRR) domain have an artificial disulfide pattern owing to truncated C-terminal construct boundaries
- NgR has previously been shown to self-associate via its LRR domain , but the structural basis of this interaction remains elusive
- Here, crystal structures of the NgR LRR with a longer C-terminal segment and a native disulfide pattern are presented
- An additional C-terminal loop proximal to the C-terminal LRR cap is stabilized by two newly formed disulfide bonds, but is otherwise mostly unstructured in the absence of any stabilizing interactions
- NgR crystallized in six unique crystal forms, three of which share a crystal-packing interface
- NgR crystal-packing interfaces from all eight unique crystal forms are compared in order to explore how NgR could self-interact on the neuronal plasma membrane