PMID: 29671580

 

    Legend: Sugar

Title : Site-Specific Profiling of Serum Glycoproteins Using N-Linked Glycan and Glycosite Analysis Revealing Atypical N-Glycosylation Sites on Albumin and α-1B-Glycoprotein

Abstract :
  1. Most serum proteins are N-linked glycosylated, and therefore the glycoproteomic profiling of serum is essential for characterization of serum proteins
  2. In this study, we profiled serum N-glycoproteome by our recently developed N-glycoproteomic method using solid-phase extraction of N-linked glycans and glycosite-containing peptides (NGAG) coupled with LC-MS/MS and site-specific glycosylation analysis using GPQuest software
  3. Our data indicated that half of identified N-glycosites were modified by at least two glycans , with a majority of them being sialylated
  4. Specifically, 3/4 of glycosites were modified by biantennary N-glycans and 1/3 of glycosites were modified by triantennary sialylated N-glycans
  5. In addition, two novel atypical glycosites (with N-X-V motif ) were identified and validated from albumin and α -1B-glycoprotein
  6. The widespread presence of these two glycosites among individuals was further confirmed by individual serum analyses