Title : Structural basis for nucleotide recognition by the ectoenzyme
CD203c
Abstract :
- The ecto-nucleotide pyrophosphatase/phosphodiesterase (NPP) enzyme family modulates purinergic signaling by degrading extracellular nucleotides
- CD203c ( NPP3 , ENPP3 ) regulates the inflammatory response of basophils via ATP hydrolysis and is a marker for allergen sensitivity on the surface of these cells
- Multiple other roles and substrates have also been proposed for this protein
- In order to gain insight into its molecular functions, we determined the crystal structure of human NPP3 as well as its complex with an ATP analog
- The enzyme exhibits little preference for nucleobase type, and forms specific contacts with the alpha and beta phosphate groups of its ligands
- Dimerization of the protein does not affect its catalytic activity
- These findings expand our understanding of substrate recognition within the NPP family
- Structural data are available in the Protein Data Bank under the accession numbers 6C01 (human NPP3 ) and 6C02 (human NPP3 T205A N594S with AMPCPP)