Title : Human urinary kallikrein--amino acid sequence and carbohydrate attachment sites
Abstract :
The amino acid sequence of human urinary kallikrein was determined by protein-chemical methods
The peptide chain contains 238 amino acid residues
Ten half-cystine residues form five disulfide bridges
Six carbohydrate side chains could be identified, three of them are N-glycosidically linked to asparagine which is present in the acceptor sequon Asn-Xaa- Thr (or - Ser ) and three are O-glycosidically linked to two serine residues and one threonine residue , respectively
Comparison of the primary structure of human urinary kallikrein with that of other serine proteinases especially porcine kallikrein and rat kallikrein shows a high degree of homology