Title : Amino acid sequences of the human kidney cathepsins H and L
Abstract :
The complete amino acid sequences of human kidney cathepsin H H (EC 3.4.22.16) and human kidney cathepsin L (EC 3.4.22.15) were determined
Cathepsin H contains 230 residues and has an Mr of 25116
The sequence was obtained by sequencing the light, heavy and mini chain and the peptides produced by cyanogen bromide cleavage of the single-chain form of the enzyme
The glycosylated mini chain is a proteolytic fragment of the propeptide of cathepsin H . Human cathepsin L has 217 amino acid residues and an Mr of 23720
Its amino acid sequence was deduced from N-terminal sequences of the heavy and light chains and from the sequences of cyanogen bromide fragments of the heavy chain
The fragments were aligned by comparison with known sequences of cathepsins H and L from other species
Cathepsins H and L exhibit a high degree of sequence homology to cathepsin B (EC 3.4.22.1) and other cysteine proteinases of the papain superfamily