Title : The N- and O-linked
carbohydrate chains of human, bovine and porcine
plasminogen
Abstract :
- Species specificity in relation to sialylation and fucosylation patterns
- The structures of the N- and O-glycans of human, bovine and porcine plasminogen were determined by 500-MHz 1H-NMR spectroscopy
- The N-glycans of all three species proved to be of the N-acetyllactosamine type differing from one another with respect to the sialylation and fucosylation patterns
- In the N-glycan of human plasminogen the two antennae are sialylated with N-acetylneuraminic acid (NeuAc), whereas in the bovine counterpart both branches carry significant amounts of N-glycolylneuraminic acid (NeuGc).
- In porcine plasminogen the sialic acid is mainly NeuAc; the Man alpha 1----6 branch, however, is only partially sialylated
- In addition, the porcine N-glycan is fucosylated to about 80% in alpha 1----6 linkage to the GlcNAc-1 residue.
- The O-glycans of the three species possess an identical Gal beta 1----3GalNAc core which is alpha 2----3 sialylated with NeuAc at Gal.
- The disialylated form , which is also present in all three species, has an additional NeuAc residue residue in alpha 2----6 linkage to GalNAc.
- Mono- and disialylated forms occur in different molar ratios in the different plasminogens : 80:20 in human, 70:30 in bovine and 50:50 in porcine
- This study on the carbohydrate moiety of these three plasminogens reveals species specificity in terms of various types of microheterogeneities