Title : Identification method for
twelve oligomannose-type sugar chains thought to be processing intermediates of
glycoproteins
Abstract :
- A mixture of the pyridylamino (PA) derivatives of 12 oligomannose-type sugar chains was fractionated into five fractions (mannose5N-acetylglucosamine2-PA approximately mannose9N-acetylglucosamine2-PA) by size-fractionation HPLC with a MicroPak AX-5 column
- Each fraction thus obtained was then analyzed by reversed-phase HPLC with a Cosmosil 5C18-P column
- In this way, the 12 PA-oligomannose-type sugar chains were completely separated from each other
- The method was used to identify the structures of oligomannose-type sugar chains of human C3, the third component of human complement