PMID: 3571235

 

    Legend: Sugar

Title : Glycophorins B and C from human erythrocyte membranes

Abstract :
  1. Purification and sequence analysis
  2. We have developed methods for the preparative purification of two sialoglycoproteins ( glycophorins B and C ) from human erythrocyte membranes by high-performance ion exchange and gel permeation chromatography in the presence of Triton X-100
  3. Glycophorin B was obtained without any detectable contaminants, and glycophorin C exhibited a purity of about 90-95%
  4. The amino acid sequence of the intramembranous domain ( residues 36-71 ) of glycophorin B was determined and found to be similar to that of the hydrophobic region of the major sialoglycoprotein ( glycophorin A )
  5. The amino acid sequence of the hydrophobic domain ( residues 49-88 ) of glycophorin C , that was also determined, agreed completely with the structure recently deduced from cDNA sequencing