Title : Structure of
sialyl-oligosaccharides isolated from bronchial mucus
glycoproteins of patients (blood group O) suffering from cystic fibrosis
Abstract :
- The carbohydrate chains of the bronchial-mucus glycoproteins of six cystic fibrosis patients with blood group O were released by alkaline borohydride treatment
- Low-molecular-mass, monosialyl oligosaccharide-alditols were isolated by anion-exchange chromatography and fractionated by high-performance liquid chromatography
- Structural characterization was performed by 500-MHz 1H-NMR spectroscopy in combination with quantitative sugar analysis
- The established structures range in size from tetra- up to heptasaccharides
- They are all sialyl analogs of neutral oligosaccharides that were characterized previously [Lamblin G., Boersma A., Lhermitte M., Roussel P., Mutsaers J. H. G. M., Van Halbeek H. & Vliegenthart J. F. G. (1984) Eur
- J. Biochem
- 143, 227-236]
- The NeuAc residue was found to occur either in alpha (2----3)-linkage to Gal, or in alpha (2----6)-linkage to GalNAc-ol or Gal.