Title : Structures of the major carbohydrates of natural human interleukin-2
Abstract :
Purified human interleukin-2 secreted by peripheral blood lymphocytes from healthy donors was found to exist in several forms
These forms were (partially) resolved by reversed-phase high-performance liquid chromatography and sodium dodecyl sulfate/polyacrylamide gel electrophoresis
Two major polypeptide species ( interleukin-2 N1 and N2, 16.5 kDa) were shown to be glycosylated on the basis of [3H]galactose/[3H]glucosamine incorporation and determination of amino sugars after acid hydrolysis
A third component ( interleukin-2 M , 14.5 kDa) represents a nonglycosylated form
The amino acid com position and the NH2-terminal sequence of both forms are consistent with the data deduced from the cDNA coding for interleukin-2 after removal of a leader peptide of 20 amino acids
Carbohydrates are O-linked to the IL-2 protein via threonine-3 of the polypeptide chain
The oligosaccharides were released by reductive beta-elimination and were purified by gel filtration and high-performance liquid chromatography
Applying methylation analysis, exoglycosidase digestion and fast atom bombardment mass spectrometry the following major carbohydrate structures were identified: N1, NeuAc(alpha 2-3)Gal(beta 1-3)GalNAc-ol; and N2, NeuAc(alpha 2-3)Gal(beta 1-3)[NeuAc(alpha 2-6)]GalNAc-ol.