PMID: 7106126

 

    Legend: Sugar

Title : N-terminal amino acid sequence of sialoglycoprotein D ( glycophorin C ) from human erythrocyte membranes

Abstract :
  1. The amino acid sequence of the N-terminal tryptic glycopeptide from a minor human erythrocyte membrane sialoglycoprotein (component D or glycophorin C ) was determined by manual sequencing
  2. The glycosylation sites were identified by a new procedure for the detection of the glycosylated derivatives released by Edman degradation
  3. The fragment , comprising 47 residues, was found to contain an average of about 12 O-glycosidically linked oligosaccharides and one asparagine-linked carbohydrate chain. asparagine-linked carbohydrate chain
  4. An identical hexapeptide sequence occurring in two regions of the glycopeptide provides evidence that it has developed by an internal gene duplication during evolution
  5. In addition, a part of its structure shows a striking similarity to the sequence of a certain region of the MN and Ss erythrocyte membrane sialoglycoproteins ( glycophorins A and B ), suggesting that the molecules might be related