Title : Studies on the structures of
the carbohydrate moiety of human
prothrombin
Abstract :
- Human prothrombin contains three asparagine-linked sugar chains asparagine-linked sugar chains in one molecule
- The sugar chains were quantitatively liberated as radioactive oligosaccharides from the polypeptide moiety by hydrazinolysis followed by N-acetylation and Nab3H4 reduction
- All of the oligosaccharides contain N-acetylneuraminic acid
- The neutral oligosaccharides obtained from all acidic oligosaccharides by sialidase digestion are identical
- By the combination of sequential exoglycosidase digestion and methylation analysis, the structures of the asparagine-linked sugar chains asparagine-linked sugar chains of human prothrombin were confirmed to be as follows: (sequence in text)