PMID: 7541354

 

    Legend: Sugar

Title : Structures of the N-linked oligosaccharides on human plasma vitronectin

Abstract :
  1. The structures of N-linked oligosaccharides present on human plasma vitronectin were elucidated
  2. Oligosaccharides were released from the vitronectin by N-glycosidase F digestion and tagged with 2-aminopyridine; the pyridylamino-oligosaccharides were then fractionated by anion-exchange and reverse-phase HPLC
  3. Ten major pyridylamino-oligosaccharides were isolated
  4. The linkages and locations of sialic acid residues were determined by desialylation with Salmonella sialidase in combination with acid
  5. The asialo forms were then analyzed by two-dimensional sugar mapping, component sugar analysis and 400-MHz 1H-NMR spectroscopy
  6. The major oligosaccharides of human vitronectin were of the diantennary N-acetyllactosamine type , with a lesser amount of the tri- and a small amount of the mono-antennary type , to which 1-3 mol sialic acid residues were linked, mostly through alpha 2-6 linkages, although alpha 2-3 linkages were also present
  7. The possibility that several binding activities of vitronectin can be ascribed to its glycan moiety was discussed, based on the specific features of the N-linked oligosaccharides on human vitronectin revealed here