PMID: 7688323

 

    Legend: Sugar

Title : Localization of an O-glycosylation site in the alpha-subunit of the human platelet integrin GPIIb /IIIa involved in Baka ( HPA-3a) alloantigen expression

Abstract :
  1. The human alloantigen system Baka/b is associated with a Ile843--> Ser replacement on platelet glycoprotein IIb , the alpha- subunit of the integrin receptor for fibrinogen ( GPIIb /IIIa)
  2. Recent immunological studies indicate that sialylated oligosaccharide chain(s) are also implicated in expression of the Baka determinant
  3. Here we show that the GPIIb fragment 704-856 contains the whole Baka epitope , and that chemical cleavage of a single O-linked oligosaccharide chain within this GPIIb domain correlates with the loss of its anti-Baka antibodies binding ability
  4. Ser847 was identified as the O-glycosylation site
  5. Therefore, our results show that the Ser847 modification is responsible for the expression of the GPIIb-specific Baka alloantigen, and provide thus a link between the molecular biology and the immunologic observations