Title : Cloning and sequence analysis of a cDNA clone coding for
the mouse GM2 activator protein
Abstract :
- A cDNA (1.1 kb) containing the complete coding sequence for the mouse GM2 activator protein was isolated from a mouse macrophage library using a cDNA for the human protein as a probe
- There was a single ATG located 12 bp from the 5' end of the cDNA clone followed by an open reading frame of 579 bp
- Northern blot analysis of mouse macrophage RNA showed that there was a single band with a mobility corresponding to a size of 2.3 kb
- We deduce from this that the mouse mRNA, in common with the mRNA for the human GM2 activator protein , has a long 3' untranslated sequence of approx
- 1.7 kb
- Alignment of the mouse and human deduced amino acid sequences showed 68% identity overall and 75% identity for the sequence on the C-terminal side of the first 31 residues , which in the human GM2 activator protein contains the signal peptide
- Hydropathicity plots showed great similarity between the mouse and human sequences even in regions of low sequence similarity
- There is a single N-glycosylation site in the mouse GM2 activator protein sequence ( Asn151-Phe-Thr ) which differs in its location from the single site reported in the human GM2 activator protein sequence ( Asn63-Val-Thr )