Title : Identification and structural characterization of
a mannose-6-phosphate containing oligomannosidic N-glycan from human
erythropoietin secreted by recombinant BHK-21 cells
Abstract :
- A sialidase resistant mono-charged N-glycan was isolated from glycosylation site I ( Asn-24 ) of recombinant human erythropoietin expressed from baby hamster kidney (BHK-21) cells and constituted approximately 2-4% of the oligosaccharide material at this glycosylation site
- Mass spectrometry and both 1- and 2-dimensional NMR techniques revealed a high mannose type structure (Man6) with a phospho-diesterbridged N-acetylglucosamine as follows: [formula: see text