Title :
Sialylation and sulfation of
the carbohydrate chains in respiratory mucins from a patient with cystic fibrosis
Abstract :
- Mucin glycopeptides were prepared from the sputum of a patient suffering from cystic fibrosis
- The carbohydrate chains were released by alkaline borohydride treatment
- Sialylated and sulfated oligosaccharide-alditols were purified by ion-exchange chromatography, gel filtration, and high performance anion-exchange chromatography
- The structures of the oligosaccharide-alditols were determined by high resolution 1H NMR spectroscopy in combination with fast atom bombardment-mass spectrometry
- Twenty-four oligosaccharides were characterized and illustrate the diversity of the carbohydrate chains of cystic fibrosis respiratory mucins
- Among these 24 oligosaccharide-alditols , 15 are novel structures
- Sialylation may occur on the C-6 of the N-acetylgalactosamine involved in the carbohydrate-peptide linkage or on a terminal galactose residue, either on C-3 or on C-6
- Sulfation may occur either on the C-3 of a terminal galactose residue or on the C-6 of a N-acetylglucosamine residue.
- The most complex structures contain sulfated derivatives of the H,X, or Y determinants or a sialylated and sulfated derivative of the X determinant (Structure A)