Title : An improved approach to the analysis of the structure of
small oligosaccharides of
glycoproteins : application to
the O-linked oligosaccharides from human
glycophorin A . Treatment of purified human
glycophorin A with alkaline borohydride cleaved
the oligosaccharide side chains to yield alditol derivatives that were separated by gel filtration into three mixtures of low molecular weight compounds
Abstract :
- Each mixture was oxidised with periodate, and the products were reduced with borohydride and analysed after acetylation or methylation by GLC-MS and FABMS
- The resulting data allowed the monosaccharide sequence and linkage positions to be assigned to each component of the mixtures
- The anomeric configuration was determined by 1H NMR spectroscopy of the intact fractions
- The structures of a desialylated tetrasaccharide, two monosialylated trisaccharides, and five other minor products were defined