PMID: 8407898

 

    Legend: Sugar

Title : Three-dimensional structure of rat acid phosphatase in complex with L(+)-tartrate

Abstract :
  1. The crystal structure of recombinant rat prostatic acid phosphatase in complex with the inhibitor L(+)-tartrate was determined to 3-A resolution with protein crystallographic methods
  2. The inhibitor binds at the carboxyl end of the parallel strands of the alpha/beta domain
  3. One of the carboxyl groups of the tartrate molecule interacts with the conserved residues Arg-11, His-12, and Arg-15 , which form part of the phosphate binding site
  4. Furthermore, the C2 and C3 hydroxyl groups interact with His-257 and Arg-79
  5. The second carboxyl group is close to Arg-79 but makes no direct hydrogen bonds to the protein
  6. A sequence comparison between tartrate-sensitive and -resistant acid phosphatases suggests that these enzymes have different three-dimensional structures