Title : The glycosylation of Bowes melanoma
tissue plasminogen activator : lectin mapping, reaction with
anti-L2/HNK-1 antibodies and the presence of
sulphated/glucuronic acid containing glycans.
Abstract :
- The glycosylation of tissue plasminogen activator ( t-PA ) obtained from the Bowes melanoma cell line was re-examined using methods of serial lectin affinity chromatography coupled with Bio-Gel P-4 gel filtration chromatography and exoglycosidase sequencing
- This study clarified an earlier discrepancy in the literature and confirmed that the major complex N-linked glycans on Bowes t-PA that carry sialic acid as their sole charged group are bi-antennary, core fucosylated, with terminal N-acetylgalactosamine residues.
- We also report the characterization of a series of related and previously unidentified sialylated glycans
- Further we show that Bowes t-PA expresses glucuronic acid/sulphate containing N-linked glycans and is recognized by anti-carbohydrate L2/HNK-1 monoclonal antibodies
- The presence on Bowes t-PA of glycans associated primarily with the nervous system is consistent with its expression in a cell line of neuroectodermal origin