PMID: 9628725

 

    Legend: Sugar

Title : Crystal structure of fragment double-D from human fibrin with two different bound ligands

Abstract :
  1. Factor XIII-cross-linked fragment D (double-D) from human fibrin was crystallized in the presence of two different peptide ligands and the X-ray structure determined at 2.3 A
  2. The peptide Gly-Pro-Arg-Pro-amide , which is an analogue of the knob exposed by the thrombin-catalyzed removal of fibrinopeptide A, was found to reside in the gamma-chain holes, and the peptide Gly-His-Arg-Pro-amide , which corresponds to the beta-chain knob, was found in the homologous beta-chain holes
  3. The structure shows for the first time that the beta-chain knob does indeed bind to a homologous hole on the beta-chain
  4. The gamma- and beta-chain holes are structurally very similar, and it is remarkable they are able to distinguish between these two peptides that differ by a single amino acid
  5. Additionally, we have found that the beta-chain domain , like its gamma-chain counterpart, binds calcium