Title : Effects of glycosylation of the
residue at position 14 in ovine
angiotensinogen on the human
renin reaction
Abstract :
- A mutant angiotensinogen , S14N, in which Ser14 of ovine angiotensinogen was replaced by Asn to form a N-glycosylation site , was produced in CHO cells
- The molecular weight was about 3,000 larger than that of wild-type ovine angiotensinogen , indicating that S14N angiotensinogen was glycosylated at Asn14
- In the reaction with human renin , the km of mutant angiotensinogen was 3 times increased, but the Vmax was not affected by the mutation