Title :
Doppel is an N-glycosylated, glycosylphosphatidylinositol-anchored
protein
Abstract :
- Expression in testis and ectopic production in the brains of Prnp (0/0) mice predisposed to Purkinje cell loss
- The Prnd gene encodes a homolog of the cellular prion protein ( PrP ( C )) called doppel ( Dpl )
- Up-regulation of Prnd mRNA in two distinct lines of PrP gene ablated ( Prnp (0/0)) mice, designated Rcm0 and Ngsk, is associated with death of Purkinje cells
- Using recombinant Dpl expressed in Escherichia coli and mouse neuroblastoma cells we demonstrate that wild type (wt) Dpl , like PrP ( C ), adopts a predominantly alpha-helical conformation, forms intramolecular disulfide bonds, has two N-linked oligosaccharides, and is presented on the cell surface via a glycosylphosphatidylinositol anchor
- Dpl protein was detected in testis of wt mice
- Using Triton X-114 phase partitioning to enrich for glycosylphosphatidylinositol-anchored proteins , Dpl was detected in brain samples from Rcm0 Prnp (0/0) mice but was absent in equivalent samples from wt mice and ZrchI Prnp (0/0) mice, indicating that ectopic expression of this protein may cause cerebellar pathology in Rcm0 mice
- Biochemical and structural similarities between PrP ( C ) and Dpl documented here parallel the observation that ataxic Ngsk Prnp (0/0) mice can be rescued by overexpression of wild-type PrP transgenes, and suggest that cell surface PrP ( C ) can antagonize the toxic effect of Dpl expressed in the central nervous system
Output (sent_index, trigger,
protein,
sugar,
site):
- 0. N-glycosylated, , Doppel, -, -
- 0. N-glycosylated, , protein, -, -
- 4. has, , Dpl, two N-linked oligosaccharides, -
Output(Part-Of) (sent_index,
protein,
site):
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):