PMID: 10988252

 

    Legend: Gene, Sites

Title : Characterization of the carbohydrate chains of the secreted form of the human epidermal growth factor receptor

Abstract :
  1. The human epidermal growth factor receptor ( EGFR ) is a transmembrane glycoprotein having 11 potential N-glycosylation sites in its extracellular domain
  2. N-Glycosylation is needed for proper membrane insertion, EGF binding and receptor functioning
  3. The human epidermoid carcinoma A431 cell line secretes a soluble 105 kDa glycoprotein ( sEGFR ) that represents the extracellular domain of the membrane-bound form, and its glycosylation pattern has been investigated
  4. After liberation of the oligosaccharides from sEGFR with PNGase F , the glycans were fractionated along different routes, including Concanavalin A affinity chromatography, anion-exchange chromatography, HPLC and high-pH anion-exchange chromatography
  5. The oligosaccharide fractions were characterized by 500- and 600-MHz 1H-NMR spectroscopy and mass spectrometry ( FAB , ESI , and MALDI-TOF)
  6. The oligomannose-type glycans range from Man5GlcNAc2 to Man8GlcNAc2 and account for 17% of the total carbohydrate moiety
  7. Furthermore, di-, tri'- and tetraantennary complex-type structures are present, both neutral and (alpha2-3)-sialylated (up to tetrasialo), comprising 24 and 59%, respectively, of the total carbohydrate moiety
  8. In this study, 32 new complex-type glycans are characterized containing the Le(x), Le(Y), and sialyl-Le(x) determinants, the bloodgroup A and H antigens, as well as the ALe(Y) determinant
  9. This first comprehensive glycosylation study on a human nonrecombinant receptor shows the immense heterogeneity of the glycosylation of sEGFR
Output (sent_index, trigger, protein, sugar, site):
  • 1. N-glycosylation, , -, -, sites
  • 1. glycoprotein, , epidermal growth factor receptor, -, -
  • 1. glycoprotein, , glycoprotein, -, -
  • 3. glycoprotein, , glycoprotein, -, -
  • 3. glycoprotein, , sEGFR, -, -
  • 4. sEGFR, , sEGFR, the oligosaccharides, -
  • 9. glycosylation, , sEGFR, -, -
Output(Part-Of) (sent_index, protein, site):
  • 1. epidermal growth factor receptor, sites
  • 1. glycoprotein, sites
*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

 

Protein NCBI ID SENTENCE INDEX
FAB 2187 5
epidermal growth factor receptor 1956 0,1
EGFR 1956 1