PMID: 11459848

 

    Legend: Gene, Sites

Title : Structural characterization of human recombinant and bone-derived bone sialoprotein

Abstract :

1. Functional implications for cell attachment and hydroxyapatite binding
2. Human bone sialoprotein ( BSP ) comprises 15% of the total noncollagenous proteins in bone and is thought to be involved in bone mineralization and remodeling
3. Recent data suggest a role for BSP in breast cancer and the development of bone metastases
4. We have produced full-length recombinant BSP in a human cell line and purified the protein from human bone retaining the native structure with proper folding and post-translational modifications
5. Mass spectrometry of bone-derived BSP revealed an average mass of 49 kDa and for recombinant BSP 57 kDa
6. The post-translational modifications contribute 30-40%
7. Carbohydrate analysis revealed 10 different complex-type N-glycans on both proteins and eight different O-glycans on recombinant BSP , four of those were found on bone-derived BSP
8. We could identify eight threonines modified by O-glycans, leaving the C terminus of the protein free of glycans
9. The recombinant protein showed similar secondary structures as bone-derived BSP
10. BSP was visualized in electron microscopy as a globule linked to a thread-like structure
11. The affinity for hydroxyapatite was higher for bone-derived BSP than for recombinant BSP
12. Cell adhesion assays showed that the binding of BSP to cells can be reversibly diminished by denaturation

Output (sent_index, trigger, protein, sugar, site):

• 7. N-glycans, , BSP, N-glycans, -
• 7. N-glycans, , proteins, N-glycans, -
• 7. O-glycans, , BSP, O-glycans, -
• 7. O-glycans, , proteins, O-glycans, -

Output(Part-Of) (sent_index, protein, site):

• 8. protein, terminus

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX
bone sialoprotein	3381	0
BSP	3381	2,3,4,5,7,9,10,11,12
Human bone sialoprotein	3381	2