Title : Differential and cell-type specific microheterogeneity of high mannose-type
Asn-linked oligosaccharides of human
transferrin receptor
Abstract :
- The structural analysis of high mannose-type Asn-linked (N-linked) oligosaccharides of the human transferrin receptor ( hTR ) from D-[2-3H]mannose metabolic-radiolabeled human cells--A431, K562, BeWo, and HL60--was investigated
- The radiolabeled hTR glycopeptides were prepared and fractionated by a lectin chromatography of Concanavalin A-Sepharose
- The com position analysis of hTR glycopeptides revealed that Con A-I contains both mannose and fucose, whereas Con A-III has mannose exclusively
- The Con A-III glycopeptides were treated with Endo H
- The released oligosaccharides were charge-fractionated by QAE-Sephadex
- The neutral oligosaccharides were further size-fractionated by an amine absorption high performance liquid chromatography (HPLC)
- Our results demonstrate that the high mannose-type oligosaccharides of hTR ranged in size from Man5-R to Man9-R with cell-type specific patterns
- A relative amount of each component was found to be differentially heterogeneous among the four different human cell lines
Output (sent_index, trigger,
protein,
sugar,
site):
- 0. Asn-linked, , -, high mannose-type Asn-linked oligosaccharides, Asn
- 0. microheterogeneity, , -, high mannose-type Asn-linked oligosaccharides, -
- 0. receptor, , transferrin receptor, high mannose-type Asn-linked oligosaccharides, -
- 1. Asn-linked, , -, high mannose-type Asn-linked (N-linked) oligosaccharides, Asn
- 1. cells--A431, , hTR, cells--A431, -
- 1. cells--A431, , transferrin receptor, cells--A431, -
- 1. receptor, , transferrin receptor, high mannose-type Asn-linked (N-linked) oligosaccharides, -
- 2. glycopeptides, , -, -, glycopeptides
- 3. glycopeptides, , -, -, glycopeptides
- 4. glycopeptides, , -, -, glycopeptides
- 7. hTR, , hTR, the high mannose-type oligosaccharides, -
Output(Part-Of) (sent_index,
protein,
site):
- 2. hTR, glycopeptides
- 3. hTR, glycopeptides
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):