PMID: 11805077

 

    Legend: Gene, Sites

Title : Structural analysis of sulfated N-linked oligosaccharides in erythropoietin

Abstract :

1. We previously demonstrated that high-performance liquid chromatography with electrospray ionization mass spectrometry (LC/MS) equipped with a graphitized carbon column ( GCC ) is useful for the structural analysis of carbohydrates in glycoproteins
2. Using LC/MS with GCC , sulfated N-linked oligosaccharides were found in erythropoietin ( EPO ) expressed in baby hamster kidney cells
3. Sulfation occurs in a part of the N-linked oligosaccharides in the EPO
4. Sulfated monosaccharide residue in the sulfated N-linked oligosaccharide was determined by exoglycosidase digestion followed by sugar mapping by LC/MS
5. The linkage position and branch-location of the sulfate group in the tetraantennary oligosaccharide were analyzed by (1)H-nuclear magnetic resonance
6. It was suggested that sulfation occurs on the C-6 position of GlcNAc located in the GlcNAcbeta1-4Manalpha1-3 branch

Output (sent_index, trigger, protein, sugar, site):

• 0. oligosaccharides, , erythropoietin, oligosaccharides, -
• 1. carbohydrates, , glycoproteins, carbohydrates, -
• 1. glycoproteins, , glycoproteins, -, -
• 2. found, , EPO, sulfated N-linked oligosaccharides, -
• 2. found, , erythropoietin, sulfated N-linked oligosaccharides, -
• 3. oligosaccharides, , EPO, oligosaccharides, -
• 4. residue, , -, the sulfated N-linked oligosaccharide, residue in
• 5. position, , -, the tetraantennary oligosaccharide, position

Output(Part-Of) (sent_index, protein, site):

• 6. C-6, position

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX